TABLE 1.

CaaX farnesylation sequence affects binding properties of Pex19p^a

BD-hybrid	Optical density
	AD-Pex19p	AD-Pex19pΔCaaX
Pex3p	163	162
Pex16p	22.2	15.1
Pex12p	0.540	0.002
Pex13p	0.431	0.004
Pex11pβ	0.268	0.030
Pex10p	0.199	0.038

^aSFY526 yeast cells, transformed with plasmids encoding one of the indicated Ga14p DNA-BD fusion proteins (BD-hybrid) and the Ga14p AD fused to either Pex19p or Pex19pΔCaaX, were selected for leucine and tryptophan prototrophy. Double transformants were assayed for β-galactosidase using o-nitrophenyl-β-d-galactopyranoside as substrate. The optical densities were measured at 421 nm and normalized for culture densities (optical density at 600 nm = 10) and time (24 h). The values given are the means of three measurements performed on independent single colonies.