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. Author manuscript; available in PMC: 2022 Aug 5.
Published in final edited form as: Cell. 2021 Jul 7;184(16):4329–4347.e23. doi: 10.1016/j.cell.2021.06.023

Figure 1. All known neuron types in the C. elegans nervous system are identified as individual clusters of scRNA-seq profiles.

Figure 1.

A) All neuron types in the mature C. elegans hermaphrodite. B) UMAP projection of 70,296 neurons with all neuron types and sub-types of ten anatomically defined classes. Neuron identities were assigned based on the expression of known marker genes (Table S1, Figure S3). C) Graphical representation of neurons targeted in individual experiments. D) (top left) The LUA cluster exclusively expressed C39H7.2. Confocal image showing expression of transcriptional reporter C39H7.2::NLS-GFP in LUA neurons (LUAL and LUAR) (arrows) in tail region of NeuroPAL strain. Scale bar = 10 μm. E) Sub-UMAP of central group of cells in B. Clusters are annotated by cell types. F) Sub-UMAP of several commingled neurons in B that clearly separates closely related neuron types (e.g., FLP vs PVD) into individual clusters. See also Figure S1, S2, S3.