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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2021 Jun 26;164(5):e207–e226. doi: 10.1016/j.jtcvs.2021.06.029

Figure 3. Mito-Tempo (MT) significantly increases SK channel currents of mouse heart endothelial cells (MHECs) in H/R model from ND and DM mice.

Figure 3.

A, Representative traces of the whole cell currents of MHECs treated with or without MT (10μM) at holding potential of −50 mV and test potentials from −100 to +100 mV in 20 mV increments. B, whole-cell I–V relationships sensitive to NS309 in MHECs of ND(H/R) and DM(H/R) with or without MT treatment. C, whole-cell I–V relationships sensitive to TRAM34+Apamin in MHECs of ND(H/R) and DM(H/R) with or without MT treatment. D, Box plots shows NS309-sensitive component of potassium current at +100 mV in ND(H/R) and DM(H/R) MHECs treat with or without MT (n=5/group). *P = 0.0074, ND(H/R) +10μM MT vs. ND(H/R); @P = 0.0162, DM(H/R) +10μM MT vs. DM(H/R). E, Box plots shows TRAM34+Apamin-sensitive component of potassium current at +100 mV in ND(H/R) and DM(H/R) MHECs treat with or without MT (n=5/group). *P =0.0322, ND(H/R) +10μM MT vs. ND(H/R); @P =0.0451, DM(H/R) +10μM MT vs. DM(H/R). ND(H/R) = ND treated by hypoxia/re-oxygenation; DM(H/R) = DM treated by hypoxia/re-oxygenation; ND(H/R) +10μM MT = ND(H/R) + 10μM mito-Tempo; DM(H/R) +10μM MT = DM(H/R) + 10μM mito-Tempo; Mean ± SD, One-way ANOVA with a post hoc Sidak's multiple comparisons test.