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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2021 Jun 26;164(5):e207–e226. doi: 10.1016/j.jtcvs.2021.06.029

Figure 4. Mito-Tempo (MT) significantly increases SK channel currents of MHECs in H/R model from ND and DM mice.

Figure 4.

A-D, Time course of the whole-cell current density evoked at +100mV from MHECs using patch clamp. NS309 was added to the bath to activated IK/SK channels, followed by bath application of Apamin and TRAM34 to block them. E, Box plots shows NS309-sensitive component of potassium current at +100 mV in ND(H/R) and DM(H/R) MHECs treat with or without MT (n=3/group); *P =0.0486, ND(H/R)+10μM MT vs. ND(H/R); @P =0.0082, DM(H/R)+10μMT vs. DM(H/R). F, Box plots shows Apamin+TRAM34-sensitive component of potassium current at +100 mV in ND(H/R) and DM(H/R) MHECs treat with or without MT (n=3/group). *P =0.0233, ND(H/R) +10μM MT vs. ND(H/R); @P =0.0203, DM(H/R) +MT vs. DM(H/R); ND(H/R) = ND treated by hypoxia/re-oxygenation; ND(H/R) +10μM MT = ND(H/R) + 10μM mito-Tempo; DM(H/R) = DM treated by hypoxia/re-oxygenation; DM(H/R) +10μM MT = DM(H/R) + 10 μM mito-Tempo; Mean ± SD, One-way ANOVA with Student's t-test.