| Component | Volume (μL) | Final Concentration |
|---|---|---|
| Cu(II)SO4 (200 mM) | 0.5 | 2 mM |
| Sulfo-Cy3-azide (4 mM) | 0.05 | 4 μM |
| Sodium ascorbate (200 mg/mL) | 5 | 20 mg/mL |
| PBS | 44.45 | |
| Total | 50 |
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