| Reagent | Volume (μL) |
|---|---|
| SensiFAST Probe Lo-Rox | 5 |
| Forward primer (100 μM) | 0.05 |
| Reverse primer (100 μM) | 0.05 |
| UPL probe | 0.1 |
| RNase free water | 2.3 |
| cDNA | 2.5 |
| Total | 10 |
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