Table 1 ∣.
Primer and probe sequences for senescence and housekeeping genes
| Primer sequence (5′–3′) | Probe sequence (5′–3′) | |
|---|---|---|
| Gene (up) | ||
| p16INK4a | F: GAGCAGCATGGAGCCTTC R: CGTAACTATTCGGTGCGTTG |
Sense: CTCCAGCA Antisense: TGCTGGAG |
| p21WAF1/Cip1 | F: TCACTGTCTTGTACCCTTGTGC R: GGCGTTTGGAGTGGTAGAAA |
Sense: GGGAGCAG Antisense: CTGCTCCC |
| GDNF | F: ATGTCCAACCTAGGGTCTGC R: CATCCCATAACTTCATCTTAAAGTCC |
Sense: CCGCCGCC Antisense: GGCGGCGG |
| TSPAN13 | F: TCAACCTGCTTTACACCTTGG R: AATCAGCCCGAAGCCAAT |
Sense: CTCCAGCA Antisense: TGCTGGAG |
| CCND1 | F: GCTGTGCATCTACACCGACA R: TTGAGCTTGTTCACCAGGAG |
Sense: AGGAGCTC Antisense: GAGCTCCT |
| DYNLT3 | F: GTGCTCTACCGGCGTGTC R: CAGCATTGAAGCCAACCTC |
Sense: CTCCTCCA Antisense: TGGAGGAG |
| PDLIM4 | F: GGATCCACATCGATCCTGAG R:GCTTGGTCTGCCATCTTCTG |
Sense: GCCTGCTG Antisense: CAGCAGGC |
| Gene (down) | ||
| LMNB1 | F: GTGCTGCGAGCAGGAGAC R: CCATTAAGATCAGATTCCTTCTTAGC |
Sense: CCCAGCAG Antisense: CTGCTGGG |
| NF1A | F: CCAGATTAGGGGGATGGTATG R: CCCCTTATCTTTCTAAGCATTTCC |
Sense: GGGAGCAG Antisense: CTGCTCCC |
| EFNB3 | F: TGGAACTCGGCGAATAAGAG R: CGATCTGAGGGTACAGCACA |
Sense: CTCTGCCT Antisense: AGGCAGAG |
| PATZ1 | F: GCAACTTCTGCAGTATCTGTAACC R: ACCGTGGTGGGTTTTAACAT |
Sense: CTCTGCCT Antisense: AGGCAGAG |
| SPIN4 | F: ACTATTTCCCTACAGCAGAACAGG R: GCTTGCCCACGAGACTGT |
Sense: CCTGGAGA Antisense: TCTCCAGG |
| CNTLN | F: AAGGCAGCAATACAAGAATTGAA R: TTGACCTCATCATCTTCACCAG |
Sense: TGGCAGAG Antisense: CTCTGCCA |
| Gene (reference) | ||
| α-tubulin | F: CTTCGTCTCCGCCATCAG R: CGTGTTCCAGGCAGTAGAGC |
Sense: GCCTGCTG Antisense: CAGCAGGC |
| β-actin | F: CCAACCGCGAGAAGATGA R: CCAGAGGCGTACAGGGATAG |
Sense: CAGCCTGG Antisense: CCAGGCTG |