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. 2021 Nov 30;148(22):dev199894. doi: 10.1242/dev.199894

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Restoration of endocytosis corrects SD delocalisation in Cubn2^E3-1 nephrocytes. (A,A′) Promoting global endocytosis by Rab5 expression in Cubn2-attenuated nephrocytes largely restores SD positioning [arrowheads indicate SD internal accumulation; arrows indicate gaps in SD-protein distribution; n=106 out of 131 nephrocytes (N), 22 strings (S) examined. (B) Megalin expression in wild-type nephrocytes [arrow indicates Mgl in the proventriculus (PV), n=29N/3S]. (C) Ectopically expressed Mgl accumulates with Cubn in the LCh membrane (arrowheads, n=20N/2S). (D-G″) Ectopic Mgl in Amnionless³ nephrocytes localises at the LCh (D, n=21N/2S,) concomitant with the ER retention of Cubn and Cubn2 (arrows in E, n=42N/4S, and F, n=18N/2S), and suppresses the Duf/Pyd internal aggregates found in Amnionless³ (G″, n=28/2S). However, note the lower density of SDs at the periphery (G, compare with Fig. 1A) and the presence of ectopic SDs at the subcortical region (G′, arrowheads in G″; inset in G″ is at 2.5× higher magnification). Scale bars: 10µm.