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PDPN was downregulated by si-PDPN. (A) The mRNA expression of PDPN in CAL-27 and CTSC-3 cells after the transfection of si-PDPN as determined by RT-qPCR. (B) The protein expression of PDPN in CAL-27 and CTSC-3 cells after transfection of si-PDPN as determined by Western blotting. CON: Blank control group; NC: Negative control; PDPN: Podoplanin; RT-qPCR: Quantitative real-time reverse transcription-polymerase chain reaction. ∗P < 0.001 vs. the blank control group.
