FIG. 3.

Features of MTAD and the RHA homologues. (A) Alignment of MTAD sequences of RHA homologues from C. elegans to humans. The conserved residues among RHA homologues are indicated with shaded boxes. Mutants with each conserved residue changed to alanine were generated. (B) Transactivation assay with the region in C. elegans corresponding to MTAD. Yeast strain Y190 cells were transformed with eMTAD inserted into pGBT9 or empty vector alone. The β-Gal activity derived from cells transformed with empty vector was designated 1. Each value of relative β-Gal activity represents the mean ± standard error (n = 3).