FIG. 3.

PLIP interacts with cPLA₂. COS cells were transfected as follows: empty pMT₃ vector alone; pEGFP-cPLA₂ and pMT₃; pEGFP-cPLA₂ and pMT₃-PLIP; and pEGFP-cPLA₂ and pMT₃-Tip60. Cell lysate proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and analyzed by Western blot analysis. Western blot with anti-cPLA₂ antibody demonstrates a band at approximately 112 kDa in lanes 6, 7, and 8, which contain lysates of pEGFP-cPLA₂-transfected COS cells (arrow). Western blot with anti-HA antibody demonstrates a band at approximately 50 kDa in lane 3, which contains the lysate of pMT₃-PLIP-transfected cells (arrow), and at 60 kDa in lane 4, which contains the lysate of pMT₃-Tip60-transfected COS cells (arrow). After immunoprecipitation of lysates with anti-HA antibody and resolution of the precipitants by SDS-PAGE, Western blot analysis with the anti-cPLA₂ antibody revealed a band corresponding to GFP-cPLA₂ in lysates of cells in which GFP-cPLA₂ was coexpressed with HA-PLIP (lane 11) and HA-Tip60 (lane 12) but not in lysates of cells transfected with pEGFP-cPLA₂ and pMT₃ (lane 10).