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Evaluation of Lt-RBD protein production in L. tarentolae. (A) Expression analyses of Lt-RBD in the concentrated supernatant of 10 engineered L. tarentolae clones. A band of approximately 35 kDa is visible using an anti–His-tag antibody. (B,C) Analysis of Lt-RBD protein expression, purified by affinity chromatography, and confirmed by Western Blotting using an anti–SARS/SARS-CoV-2 Coronavirus Spike Protein antibody (B) and by SDS-PAGE with Coomassie staining (C).
