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. 2021 Sep 10;6(1):209–222. doi: 10.1002/hep4.1724

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© 2021 The Authors. Hepatology Communications published by Wiley Periodicals LLC on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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FIG. 2 — SLC45A2‐AMACR is localized in the lysosome membrane. (A) Immunoblotting of SLC45A2‐AMACR‐FLAG using an antibody specific for FLAG in NIH3T3 (NSLAM) and HUH7 (HUH7SLAM) cells transformed with pCDNA4‐SLC45A2‐AMACR‐FLAG/pCDNA6TO, treated with or without tetracycline. Antibodies specific for GAPDH are for the loading control. (B) Colocalization of SLC45A2‐AMACR‐FLAG and the lysosome resident protein LAMP1 in NSLAM and HUH7SLAM cells using an antibody specific for FLAG and LAMP1 proteins (20x images). (C) Lysosomes, mitochondria, and nucleus fractionation of SLC45A2, AMACR, and SLC45A2‐AMACR proteins from HUH7, NSLAM, and HUH7‐AMD (HUH7 transformed with pCDNA4‐ΔAMACR^aa84‐394‐FLAG) cells. The antibody for each immunoblot is indicated. Abbreviations: H3, histone H3; Mit, mitochondria; Nuc, nucleus; PDH, pyruvate dehydrogenase.