FIG. 2.

Fur4p-GFP trafficks from the plasma membrane to the vacuole lumen in pep4Δ cells. Strains W3031B/D (WT) and MOB100 (pep4Δ) cotransformed with pFL38-FUR4-GFP (2μm URA3 GAL10-FUR4) and YEp96 (2μm TRP1 CUP1-UB) were grown at 30°C in YNB with galactose as the carbon source. CuSO₄ (100 μM) was added for 1 h. CHX was added to cells in exponential growth. Aliquots were withdrawn at the times indicated after addition of CHX, washed with ice-cold azide, and examined by Nomarski optics and for GFP fluorescence.