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. 2021 Nov 18;20(1):226–237. doi: 10.1111/pbi.13708

Figure 6.

Figure 6

Penetration of dsRNA‐ERG to fruit pulp and down‐regulation of gene expression. Grapes were treated with water (control) or dsRNA‐ERG followed by spray inoculation with B. cinerea. Peel and pulp samples for RNA extraction were taken 8, 24 and 48 h post‐inoculation. (a) Relative abundance of the applied dsRNA‐ERG construct was evaluated by qPCR and normalized to the actin gene of grape at 8, 24 and 48 h post‐infection. (b) Relative expression of erg11 gene at 8, 24 and 48 h post‐infection, normalized to actin gene of B. cinerea in the grape peel or pulp. (c) Relative expression of the three targeted genes by the dsRNA‐ERG and two non‐targeted genes in the ergosterol biosynthesis pathway, 48 h post‐inoculation in peel tissue. The data shown are means and standard errors. Asterisks indicate statistically significant differences (P < 0.05).