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. 2021 Sep 29;20(1):183–200. doi: 10.1111/pbi.13705

Figure 4.

Figure 4

Overexpression of PtrERF9 improved cold tolerance of transgenic plants. (a) Phenotypes of wild‐type (WT) and PtrERF9 overexpression transgenic tobacco lines (#2 and #4) in response to cold treatment. Scale bars = 1 cm. (b) Survival rate of transgenic tobacco plants after the growth recovery for 3 d at ambient environment. (c, d) Electrolyte leakage (c), MDA content (d) of transgenic tobacco plants before and after the growth recovery for 3 d at 25 °C. (e, f) Chlorophyll fluorescence (e) and F v/F m ratios (f) of transgenic tobacco plants before and after cold treatment and the growth recovery for 3 d at 25 °C. (g) Phenotypes of WT and PtrERF9 overexpression transgenic lemon plants (#15 and #18) before and after cold treatment. Scale bars = 1 cm. (h‐k) Electrolyte leakage (h), MDA content (i), chlorophyll fluorescence (j) and F v/F m (k) of WT and transgenic lemon before and after the cold treatment. Error bars represent ± SE (n = 3). Asterisks indicate significant difference between WT and the transgenic lines in response to cold treatment (***P < 0.001).