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. 2021 Sep 29;20(1):183–200. doi: 10.1111/pbi.13705

Figure 10.

Figure 10

PtrERF9 regulates ethylene synthesis via controlling ACS expression in trifoliate orange for cold response. (a–c) ACS1 expression (a), ACS activity (b) and ACC content (c) in TRV control and PtrERF9‐silencing trifoliate orange. (d–f) ACS1 expression (d), ACS activity (e) and ACC content (f) in wild‐type and PtrERF9 overexpression transgenic lemon plants. Error bars indicate ± SE (n = 3). Asterisks indicate significant differences between different groups under the same growth condition (**P < 0.01; ***P < 0.001). (g) Schematic diagram of ClACS1 promoter, black rectangle indicated the GGTGGC motif in the partial promoter fragment (P3). (h) Prey and bait constructs were used for yeast one‐hybrid analysis. pGADT7‐PtrERF9 and pGADT7‐ClERF9 were used as prey. P3 fragment was used as bait. (i) Growth of yeast cells co‐transformed with prey (PtrERF9, ClERF9) and bait (P3) on selective medium added with or without AbA. positive control (p53‐AbAi + pGAD‐p53), negative control (bait + pGADT7).