Fig. 2.

Effects of 1 on Nrf2 translocation. (A) ICP-MS quantification of cell uptake for 1. LO2 cells were incubated with 5 μM of 1 at different times. Error bars represent the standard deviations of the results from three independent experiments. P values were calculated using a one-way ANOVA with Tukey's multiple comparison test. **P < 0.01 vs. 45 min group. NS (not significant, P > 0.05) vs. 45 min group. (B) ICP-MS comparison of 1 in the nucleus and cytoplasm from LO2 cells. (C) Interactions between Keap1 and Nrf2 in LO2 cells were examined by Western blotting and co-IP. This experiment was repeated twice with similar results. (D) 1 induces nuclear translocation of Nrf2 in LO2 cells. (E) Western blot analysis of Nrf2 in the nucleus and cytoplasm in LO2 cells. This experiment was repeated twice with similar results.