Fig. 3.

Complex 1 engages Keap1 in cellulo. (A and B) Thermal stabilization of Keap1 and Nrf2 by 1 and densitometry analysis. Proteins were detected by Western blotting using the corresponding antibodies. Error bars represent the standard deviations of the results from three independent experiments. *P < 0.05 vs. DMSO group. (C and D) ITC thermograms showing titration of 200 μM of 1 into (C) Keap1 (20 μM) and (D) Nrf2 (20 μM). (E) Docking pose of complex 1 with Keap1 (PDB code: 4L7B) which is depicted as a space-filling representation showing carbon (yellow), nitrogen (blue), chlorine (green) atoms. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)