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. 2021 Dec 27;414(5):1773–1785. doi: 10.1007/s00216-021-03846-y

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© The Author(s) 2021

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Fig. 5 — Representative graph showing the uncertainty values. The relative standard deviations (RSD) were calculated per four assays targeting RdRp, E, N, and S genes for each of the four components: between-bottle RSD (white), method repeatability (gray), partitioning volume (dotted), and manual thresholding (black)