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. 2021 Dec 27;414(5):1773–1785. doi: 10.1007/s00216-021-03846-y

Table 2.

Selected SARS-CoV-2 gene location inserted in the RM

Gene Protein Start (bp) End (bp) Location inserted in RM (bp) Fragments Virus particle
ORF1ab Leader protein 266 805 417–1899 A1 Lenti-A
Nsp2 806 2719
Nsp3 2720 8554 3094–3360 A2
RNA-dependent RNA polymerase (RdRp) 13,442 16,236 13,291–13,560 A3
14,700–15,950 A4
3′-to-5′ exonuclease 18,040 19,620 18,577–19,051 A5
S Spike glycoprotein 21,563 25,384 21,363–26,001 B1 Lenti-B
ORF3a ORF3a protein 25,393 26,220
E Envelope protein 26,245 26,472 25,801–28,200 A6 Lenti-A
M Membrane glycoprotein 26,523 27,191
ORF8a ORF8 protein 27,894 28,259 27,952–29,873 B2 Lenti-B
N Nucleocapsid phosphoprotein 28,274 29,533
ORF10 ORF10 protein 29,558 29,674