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. Author manuscript; available in PMC: 2022 Mar 8.
Published in final edited form as: Nature. 2021 Sep 8;597(7877):571–576. doi: 10.1038/s41586-021-03897-2

Extended Data Figure 4 |. Cryo-EM data processing for the MIPS521-ADO-A1R-Gi2 (a) and ADO-A1R-Gi2 (b) complexes.

Extended Data Figure 4 |

Representative cryo-EM micrographs of each of the complexes. Reference-free 2D class averages of the complexes in LMNG and CHS detergent micelles. Gold-standard Fourier shell correlation (FSC) curves, showing the overall nominal resolution of 3.2 Å and 3.3 Å, respectively, at FSC 0.143. Corresponding 3D cryo-EM maps coloured according to local resolution estimation (Å) in Relion. c, Atomic resolution model of representative regions from the MIPS521-ADO-A1R-Gi2 structure of the A1R transmembrane domain, ADO, and MIPS521. The molecular model is shown in ball and stick representation, coloured by heteroatom, and the cryo-EM map displayed in mesh contoured at 0.02.