TABLE 1.
Synthetic wild-type and linker mutant oligonucletides used
| Oligonucleotide | Sequencea |
|---|---|
| MHS-70 | 5′-GGACTCTCTCTAACTCGTATTTTTTTTGCTAGAGCGAGAGAGCAGCGTAAAAAGAGCGTGCGCATCTCTC-3′ |
| MHH-60 | 5′-GGACTCTCTCTAACTCGTATTTTTTTTGCTAGAGCGAGAGAGCAGCGTAAAAAGAGCGTG-3′ |
| MHH-1/5 | 5′-GGTGGAACGCCAACTCGTATTTTTTTTGCTAGAGCGCGATCGCAGCGTAAAAAGAGCGTG-3′ |
| LS-1/9 | 5′-GGATGGAAGCTAACTCGTATTTTTTTTGCTAGAGCGAGAGAGCAGCGTAAAAAGAGCGTGCGTGCTAGCG-3′ |
| LS-3/6-7 | 5′-GGACTCTCTCTAACTCGACGCCTAGCCTGAAGAGCGAGAGAGCAGATCGCTAGCCTGCTGCGCATCTCTC-3′ |
| LS-3/5 | 5′-GGACTCTCTCTAACTCGACGCCTAGCCTGAAGAGCGCGATCGCAGCGTAAAAAGAGCGTGCGCATCTCTC-3′ |
| LS-4/6/8 | 5′-GGACTCTCTCTAACTCGTATTTTTTTTGCGGACATGAGAGAGAGTGTGAAAACACTGTAACCCATCTCTC-3′ |
| (GA)3 × 3 | (5′-TCTCTCagcgAGAGAGagcgTCTCTC-3′)3 |
| (A)8 × 5 | (5′-ATTTTTTTTGCTAGAGCG-3′)5 |
| (T)5 × 9 | (5′-TAAAAAGAGCG-3′9 |
a
Nucleotide substitutions are shown in bold uppercase. Nucleotides shown in bold lowercase in (GA)3 × 3 represent spacer sequences.