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. 2021 Dec 13;11:789885. doi: 10.3389/fonc.2021.789885

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Copyright © 2021 Ribelli, Simonetti, Iuliani, Rossi, Vincenzi, Tonini, Pantano and Santini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AR and AR-target gene expression in C4-2B FR cells after OCM treatment. (A) AR mRNA levels normalized for the housekeeping β-glucoronidase (GUS-β). Values are expressed as fold change relative to the control. (B) Representative image and schematic representation of AR protein expression normalized for the housekeeping β-actin. (C) AR activity of C4-2B FR cells measured as firefly luciferase signal normalized with Renilla luciferase signal (housekeeping control). (D) KLK3 and TMPRSS2 mRNA levels were normalized for the housekeeping β-glucoronidase (GUS-β). Values are expressed as fold change relative to the control.*p < 0.05; **p < 0.001.