Figure 5.

AR activity C4-2B FR cells and proliferation analysis of C4-2B GFP+ cells in “direct” co-culture with OBs. (A) AR activity measured as firefly luciferase signal normalized with Renilla luciferase signal (housekeeping control). (B) Representative images of direct co-cultures captured at 24, 48, 96, and 120 h after C4-2B GFP+ cell (green) seeding; scale bar = 100 μm. Proliferation curves C4-2B GFP+ cells co-cultured with OBs (red) or C4-2B as control (blue). GFP+ signal at each time point was normalized with GFP signal at baseline *p < 0.05; **p < 0.001.