FIG. 1.

MyD88, IRAK-1, and TRAF-6 but not IRAK-2 drive p65-mediated transactivation of gene expression. EL4.NOB-1 cells (7 × 10⁶) were transiently transfected with plasmids encoding wild-type (A) MyD88, (B) IRAK-1, and (C) TRAF-6 as indicated, along with 2.5 μg of β-galactosidase, 2.5 μg of Gal4-p65^(1-551), and 5 μg of Gal4-luciferase. Following stimulation with IL-1 (10 ng/ml), extracts were prepared and measured for luciferase activity. Results are normalized for β-galactosidase activity and are represented as fold increase over the nonstimulated empty vector (EV) control (C+l). (D) The effect of transfecting wild-type-encoding plasmids on Gal4-p65^(1–551) expression was determined by Western blotting of lysates of cells transfected with 5 μg of MyD88, IRAK-1, and TRAF-6 as indicated.