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. 2021 Dec 13;5(3):e202101112. doi: 10.26508/lsa.202101112

Figure 3. The lower vasculogenic potential of flt3L−/− side population (SP)-CPCs reflects in a decreased microvascular density and function of flt3L−/− hearts.

Figure 3.

(A) Differences in endothelial marker gene expression in EM1 (black bars) and EGM2 (blue bars) versus wt EM1, n = 3, unpaired t test. (B) Freshly isolated wt and flt3L−/− SP-CPCs were cultured in EGM2 for 3 wk and vWF-positive cells were quantified, n = 6, unpaired t test, P = 0.52. Representative pictures are shown: vWF (red), nuclei (blue). (C) Analysis of capillaries per myocyte in the left ventricular myocardium of wt versus flt3L−/− hearts, n = 9 and 10, unpaired t test. Representative pictures are shown: IB4 (red), WGA (green). (D) Left ventricular ejection fraction (LVEF) in 12-wk-old wt and flt3L−/− mice, n = 16 and 18, Mann–Whitney U test. All data are given as mean ± SEM. (E) Proposed model of Flt3 regulation of SP-CPCs.