Figure 3. Nup210 ablation results in delayed muscle regeneration.

(A) Young (6–8 wk old) Nup210^+/+ and Nup210^−/− mice were subjected to BaCl₂-induced muscle injury and muscle regeneration was analyzed 5 d later. Top: Schematic illustration of experimental approach. Bottom: Quantification of myofiber cross-sectional area distribution. n = 3, data are binned in 250 μm² bins and are plotted as mean. (B) Nup210^+/+/Pax7-CreER^T2 and Nup210^f/f/Pax7-CreER^T2 mice carrying a Cre-inducible tdTomato reporter (R26Td) were treated with tamoxifen before being subjected to BaCl₂ muscle injury. The tdTomato-positive myofibers were analyzed by immunofluorescence in muscle sections. Laminin was used as counterstain to detect muscle fibers. Top: Schematic representation of experimental approach. Bottom: Representative immunofluorescence images from TA sections. Scale bar, 100 μm. (C) Young Nup210^+/+ and Nup210^−/− mice were subjected to BaCl₂-induced serial muscle injury (three injuries total), and muscle regeneration was analyzed 25 d after last injury. Top: Schematic illustration of experimental approach. Bottom: Quantification of myofiber cross-sectional area distribution. n = 3, data are binned in 250-μm² bins and are plotted as mean.