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Selectivities of species-specific molecular beacons for reference Candida strains. In this experiment, species-specific molecular beacons, as indicated in the panels, were used to probe individual DNAs from the four Candida species. Real-time PCR amplification of the ITS2 regions of four reference Candida strains was performed with 100 ng of beacons and ∼200 ng of genomic DNA for 40 cycles in a Prism 7700 96-well spectrofluorometric thermal cycler (PE Applied Biosystems).
