Chemical similarity enrichment analysis (ChemRICH) and enrichment statistics plot for NAFL vs. HC in CAU (top panel) and HIS (bottom panel). Each cluster represents altered chemical class of metabolites (p <0.05). Cluster sizes represent the total number of metabolites. Cluster’s color represents the directionality of metabolite differences: red – higher in NAFL; blue – lower in NAFL. Colors in between refer to mixed population of metabolites manifesting both higher and lower levels in NAFL when compared to the control. The x-axis represents the cluster order on the chemical similarity tree. The plot y-axis shows chemical enrichment p-values calculated using Kolmogorov–Smirnov test. Only clusters with p <0.05 are shown. FDR-adjustment q =0.2, and clusters with FDR-adjusted p ≥0.2 are shown in gray. (n, NAFL-HIS =7, HC-HIS =14; NAFL-CAU =8, HC-CAU =8). The detailed ChemRICH results are shown in (Table S1 and S2). AC, Acylcarnitines; CAU, White Caucasian; Cer, Ceramides; CE, Cholesteryl ester; DG, Diglycerides; Ether-PL, Ether-linked phospholipids; HIS, Hispanic; LPC, Lysophosphatidylcholine; LPE, Lysophosphatidylethanolamines; MUFA, Monounsaturated fatty acid; PC, Phosphatidylcholines; PUFA, Polyunsaturated fatty acids; SFA, Saturated fatty acids; SM, Sphingomyelins; NAFL, Steatosis; TG, Triglycerides.