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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Osteoarthritis Cartilage. 2021 Oct 21;30(1):160–171. doi: 10.1016/j.joca.2021.10.004

Figure 3. Berberine exerted chondroprotective effect through activation of AMPK signaling via SIRT1 and SIRT3 in mice.

Figure 3.

Mouse knee sections from the sham control and DMM-operated mice (WT) with and without berberine chloride (BBR) treatment (as described in the figure legend in Figure 2) were used for IHC analysis. A. Representative IHC images of phosphorylated AMPKα (Thr172), total AMPKα, SIRT1 and SIRT3. B. The cells present in the non-calcified regions of femoral and tibial cartilage were subjected to further analysis. Cells with positive staining for phosphorylated and total AMPKα, SIRT1 and SIRT3 were quantified (n=3). One-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis (comparing between the sham and DMM and DMM+BBR groups). The data were expressed as mean with 95% CI. Only p values with significance were indicated in the figures.