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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Nat Chem Biol. 2021 Dec 21;18(1):8–17. doi: 10.1038/s41589-021-00903-6

Figure 3:

Figure 3:

Site-specific chemical tools for O-GlcNAc studies. a) Total protein synthesis of tau and a-synuclein specific O-GlcNAc glycoforms. b) Targeted OGT and OGA tools enable selective GlcNAc glycosylation (or de-glycosylation) of a desired protein. c) Metabolic labeling and intracellular installation of fluorescent sugar tags allows quantification of O-GlcNAcylation of a protein of interest fused to a fluorescent protein. d) O-GlcNAc roles at genomic loci are uncovered using dCas9 nuclease targeting.