Fig. 6: Context-dependent functions of TCF1 in naïve and activated CD4 and CD8 T cells.

a: Chromatin accessibility changes between naïve and activated CD4 and CD8 T cells at ATAC-seq peaks bound by TCF1 with or without Ets1. All peaks denotes peaks with >100 reads in one or more cell types.

b: TCF1 CUT&RUN signal at ATAC-seq peak regions in naïve and activated CD4 and CD8 T cells. Data points are scaled according to the fold change in chromatin accessibility and colored blue or red according to the directionality of the accessibility change. A subset of peaks that has strong preferential binding of TCF1 in activated T cells was identified in CD4 T cells and highlighted in green.

c: Chromatin accessibility changes between naïve and activated CD4 and CD8 T cells at Ets1⁺TCF1⁻ ATAC-seq peaks bound by 0–5 activation-induced TFs. Analysis was restricted to peaks with lower TCF1 binding in activated vs naïve CD8 T cells.

d: Overlap between TCF1^up peaks (green peaks in panel b) and Ets1-bound ATAC-seq peaks (top) or Ets1^up peaks (bottom).

e: Fraction of TCF1⁺Ets1⁻ peaks and TCF1^up peaks bound by 0–5 activation-induced TFs.

f: Chromatin accessibility changes in naïve and activated CD4 and CD8 T cells at TCF1^up peaks bound by 0–5 activation-induced TFs.

g: Fraction of TCF1⁺Ets1⁻ peaks and TCF1^up peaks co-bound by 0–5 activation-induced TFs that are bound by Brg1 in activated CD8 T cells (left). Mean Brg1 count at the corresponding peak sets (right).

h: Gene ontology term enrichment analysis for genes nearest to TCF1^up peaks (green dots in panel b).

i: Model of TF activity at TCF1-bound regulatory elements.