Table 1. Taxonomic affiliations of the isolates based on analysis of their 16S rRNA gene sequences.
TGB1 | TGB2 | TGB3 | TGB4 | TGB5 | TGB6 | TGB7 | ||
Accession numbers | OK287066 | OK287067 | OK287068 | OK287069 | OK287070 | OK287071 | OK287072 | |
Taxonomy | ||||||||
Annotationa | Genus | Priestia | Citrobacter | Pseudomonas | Stenotrophomonas | Burkholderia | Providencia | Micrococcus |
Hierarchy | Family | Bacillaceae | Enterobacteriaceae | Pseudomonadaceae | Xanthomonadaceae | Burkholderiaceae | Morganellaceae | Micrococcaceae |
Class | Bacillales | Enterobacterales | Pseudomonadales | Xanthomonadales | Burkholderiales | Enterobacterales | Micrococcales | |
Order | Bacilli | Gammaproteobacteria | Gammaproteobacteria | Gammaproteobacteria | Betaproteobacteria | Gammaproteobacteria | Actinomycetia | |
Phylum | Firmicutes | Proteobacteria | Proteobacteria | Proteobacteria | Proteobacteria | Proteobacteria | Actinobacteria | |
Sequence analysis | ||||||||
BLASTb | Top hitc (AN) |
Priestiad megaterium (MT509997.1) |
Citrobacter freundii (MN420979.1) |
Pseudomonas aeruginosa (KY913809.1) |
Stenotrophomonas maltophilia. (MN732977.1) |
Burkholderia contaminans (MW198159.1) |
Providencia stuartii (CP048621.1) |
Micrococcus luteus (MT533939.1) |
Query cover | 100% | 100% | 100% | 100% | 100% | 100% | 100% | |
Percent identity | 99.1% | 100% | 100% | 99.83% | 100% | 99.67% | 100% | |
RDP | SeqMatch | Bacillus | Citrobacter | Pseudomonas | Stenotrophomonas | Burkholderia | Providencia | Micrococcus |
Score | 0.957 | 1.0 | 0.998 | 0.991 | 0.998 | 0.986 | 1.0 | |
Silva ACT | Taxonomy | Bacillus | Citrobacter | Pseudomonas | Stenotrophomonas | Burkholderia | Providencia | Micrococcus |
Identity | 98.92 | 99.15 | 99.84 | 99.83 | 99.65 | 98.33 | 99.81 | |
Score | 98 | 99 | 99 | 99 | 99 | 99 | 99 | |
EzBioCloud (Type strains) | Top Hitd |
Priestia megaterium NBRC 15308 |
Citrobacter europaeus 97/99 |
Pseudomonas aeruginosa JCM 5962 |
Stenotrophomonas pavanii DSM 2513 |
Burkholderia contaminans LMG 233 |
Providencia thailandensis C1112 |
Micrococcus luteus NCTC 26 |
Similarity | 99.82% | 99.32% | 99.51% | 99.66% | 99.65% | 99.83% | 98.86% |
a Based on query cover, % identity, number of hits in BLAST, and results of RDP, Silva and EzBioCloud; b E-values were 0.0 in all BLAST results; c Accession numbers (AN) are given inside parentheses; d previously known as Bacillus megaterium.