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. 2021 Dec 14;11:786929. doi: 10.3389/fcimb.2021.786929

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Copyright © 2021 Rodriguez-Urretavizcaya, Pascual, Pastells, Martin-Gomez, Vilaplana and Marco

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Matrix effect of the Mueller–Hinton (MH) broth undiluted and diluted 5, 10, and 20 times with PBST on the (A) PYO mAb122/PC1-BSA and (B) As230/PC1-BSA ELISA. The calibration curves were run using the conditions established for the assay in PBST. The results demonstrate that it is possible to perform direct measurements in MH media diluted from 5 to 20 times in PBST for both phenazines (see Table 3 for analytical parameters of the standard curves in PBST and in 1/20 MH on PYO mAb122/PC1-BSA and Table 4 for analytical parameters of the standard curves in PBST and in 1/5 MH). The results shown are the average and standard deviations of analysis made by triplicates.