Table 3.
Features of the PYO mAb122/PC1-BSA ELISA in PBST and MH broth diluted 20 times.
| PBST | MH 1/20 | |
|---|---|---|
| Amin | 0.07 ± 0.01 | 0.05 ± 0.01 |
| Amax | 1.08 ± 0.09 | 0.92 ± 0.14 |
| Slope | -1.40 ± 0.49 | -1.11 ± 0.21 |
| IC50/nM | 0.68 ± 0.10 | 1.18 ± 0.24 |
| Dynamic range | 0.18 ± 0.08 and 2.18 ± 0.19 | 0.32 ± 0.10 and 4.13 ± 1.35 |
| LoD | 0.07 ± 0.04 | 0.15 ± 0.07 |
| R2 | 0.99 ± 0.01 | 0.99 ± 0.01 |
Assay conditions used were PC1-BSA at 0.125 µg/ml and PYO mAb122 at 0.008 µg/ml in both cases. The data shown correspond to the average of the parameters of the calibration curves performed on three different days using at least three well replicates per concentration.