Table 4.

Features of the As230/PC1-BSA ELISA in PBST and MH broth diluted five times.

	PBST	MH 1/5
Amin	0.12 ± 0.02	0.11 ± 0.02
Amax	0.99 ± 0.02	0.89 ± 0.02
Slope	-0.75 ± 0.07	-0.90 ± 0.11
IC50/nM	0.93 ± 0.05	1.02 ± 0.06
Dynamic range	0.17 and 6.47	0.24 and 5.85
LoD	0.06	0.10
R2	0.99 ± 0.03	0.99 ± 0.02

Assay conditions used were PC1-BSA at 0.0625 µg/ml and 1/6,000 dilution of As230 in both cases. The data shown correspond to the average of the parameters of the calibration curves performed on the same day using at least three well replicates per concentration.