TABLE 1.

Oligonucleotide primers used for ureA detection and vacA genotyping

Region detected	Primer designation	Primer sequence	Size of PCR product (bp)	Reference
ureA	HPU1	5′-GCCAATGGTAAATTAGTT-3′	411	10
	HPU2	5′-CTCCTTAATTGTTTTTAC-3′
s1 and s2	VA1-F	5′-ATGGAAATACAACAAACACAC-3′	259	2
	VA1-R	5′-CTGCTTGAATGCGCCAAAC-3′	286
s1a	SS1-Fa	5′-GTCAGCATCACACCGCAAC-3′	190	2
s1b	SS3-Fa	5′-AGCGCCATACCGCAAGAG-3′	187	2
s1c	S1C-Fa	5′-CTYGCTTTAGTRGGGYTA-3′	213	37
m1	VA3-F	5′-GGTCAAAATGCGGTCATGG-3′	290	2
	VA3-R	5′-CCATTGGTACCTGTAGAAAC-3′
m2	VA4-F	5′-GGAGCCCCAGGAAACATTG-3′	352	2
	VA4-R	5′-CATAACTAGCGCCTTGCAC-3′
m hybrid	VA7-Fb	5′-GTAATGGTGGTTTCAACACC-3′	705	4
	VA7-Rb	5′-TAATGAGATCTTGAGCGCT-3′

^aUsed with primer VA1-R. 

^bUsed with primers VA4F and VA4R.