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SERS-based LFA: (A) Preparation of double-layered DTNB-SiO2@AgNPs by PEI-mediated absorption method followed by addition of DNTB. (B) S protein conjugation with SiO2@Ag SERS tags. (C) LFA assembled with S protein-SiO2@Ag SERS tags drop casted onto the conjugate pad and 2 test lines coated with IgM, IgG, and control line coated with S protein Ab. The sample was applied on the sample pad and SERS signal intensities were recorded.
