Table 2.
Primers available for different target genes of SARS-CoV-2 for RT-PCR.
| Detection Method | Target Gene | Forward Primer | Reverse Primer | Reference |
|---|---|---|---|---|
| TaqMan RT-PCR | Nucleocapsid | TAATCAGACAAGGAACTGATTA | CGAAGGTGTGACTTCCATG | (38) |
| TaqMan RT-PCR | RdRp/Helicase | CGCATACAGTCTTRCAGGCT | GTGTGATGTTGAWATGACATGGTC | (39) |
| TaqMan RT-PCR | Spike | CCTACTAAATTAAATGATCTCTGCTTTACT | CAAGCTATAACGCAGCCTGTA | (39) |
| TaqMan RT-PCR | Nucleocapsid | GCGTTCTTCGGAATGTCG | TTGGATCTTTGTCATCCAATTTG | (39) |
| TaqMan RT-PCR | RdRp gene | GTGARATGGTCATGTGTGGCGG | CARATGTTAAASACACTATTAGCATA | (40) |
| TaqMan RT-PCR | Envelope | ACAGGTACGTTAATAGTTAATAGCGT | ATATTGCAGCAGTACGCACACA | (40) |
| TaqMan RT-PCR | Nucleocapsid | CACATTGGCACCCGCAATC | GAGGAACGAGAAGAGGCTTG | (40) |
| TaqMan RT-PCR | ORF1b | TGGGGYTTTACRGGTAACCT | AACRCGCTTAACAAAGCACTC | (40) |
| TaqMan RT-PCR | NSP2 | ATGCATTTGCATCAGAGGCT | TTGTTATAGCGGCCTTCTGT | (41) |