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. 2021 Dec 14;9:726931. doi: 10.3389/fcell.2021.726931

FIGURE 4.

FIGURE 4

PCK2 promotes inflammatory responses in Kupffer cells. (A) The enzyme activity of PCK2 was measured after 3MP treatment in Kupffer cells. (B) The mRNA expression of cytokines including IL-6, MCP1, and IL-10 was measured by real-time PCR. (C) The production of cytokines in the medium was measured by cytometric bead array (CBA) after 3MP treatment. (D) The protein expression of PCK2 was determined by Western blot after siRNA knockdown. (E) The mRNA expression of IL-6 and IL-10 was measured by real-time PCR after PCK2 knockdown. (F) The production of cytokines was determined by CBA after PCK2 knockdown. (G) The protein expression of PCK2 was determined by Western blot after vector transfection. (H) The mRNA expression of cytokines including IL-1β, IL-6, TNFα, and IL-10 was measured by real-time PCR. (I) The production of cytokines was determined by CBA after PCK2 overexpression (PCK2 OE). Values are mean ± SEM. *p < 0.05, **p < 0.01. values with different superscript letters mean significant differences.