FIGURE 6.

PCK2 promotes inflammation via modulating protein phosphorylation of NF-κB and AKT/MAPK signaling pathways. (A) Representative Western blots and quantification of p-AKT, AKT, p-p38, p38, p-ERK, and ERK. (B) Representative Western blots showing levels of p-AKT and p-ERK in Kupffer cells after PCK2 knockdown. (C) Representative Western blots showing levels of p-AKT and PCK2 in Kupffer cells after AKT inhibitor MK2206 treatment. (D) Phosphorylation of ERK and AKT were measured by immunoprecipitated (IP) with anti-phosphoserine and immunoblotted (IB) with anti-ERK and anti-AKT antibodies. (E) Phosphorylation of P65 NF-κB was measured by immunoprecipitated (IP) with anti-phosphoserine and immunoblotted (IB) with anti-P65 antibody. Values are mean ± SEM, *p < 0.05, **p < 0.01.