| Antibodies |
|
|
|
| Mouse monoclonal anti-CARMILI |
Abcam |
Cat. # 52681 |
| Rabbit polyclonal anti-IL-1R1 |
Abcam |
Cat. #106278 |
| Rabbit monoclonal anti-IRAK1 |
Abcam |
Cat. #180747. RRID EPR18630
|
| Rabbit monoclonal anti- phospho-p44/42 MAPK |
Cell Signaling |
Cat. # 4376S |
| Rabbit polyclonal anti-p44/42 MAPK |
Cell Signaling |
Cat. #9102 |
| Rabbit polyclonal anti-3/4 collagen fragment |
Immunoglobe |
Cat. #0217–050 |
| Mouse monoclonal anti-β-actin |
Sigma-Aldrich |
Cat. #A5441 |
|
| Chemicals, Peptides, and Recombinant Proteins |
|
|
|
| Recombinant Human IL-1β |
R&D |
Cat. #201-LB |
| Bovine plasma fibronectin |
Sigma-Aldrich |
Cat. # F4759 |
| Type I bovine collagen |
Advanced Biomatrix |
Cat. #5010 |
| Cell-penetrating TAT- Control peptide (GRKKRRQRRRPQNGAGYEMGVCVENRKVLCRSKPCNRA-Lys-FITC |
Biomatik and this paper |
Control peptide |
| LRR peptide (GRKKRRQRRRPQTL. VHLDLSGNVLRGDDLSHMYNFLAQPNK-Lys- FITC |
Biomatik and this paper |
LRR peptide |
| CP peptide (GRKKRRQRRRPQGVSFDLPAQADTLHSANKSRVKMRGKRRPQTRA-Lys- FITC |
Biomatik and this paper |
CP peptide |
|
| Experimental Models: Cell Lines |
|
|
|
| CARMIL1 KO human gingival fibroblasts |
Applied Biological Materials and this paper |
N/A |
| Wild type TERT-immortalized human gingival fibroblasts. (hTERT Cells) |
Applied Biological Materials |
T0026 |
| Human gingival fibroblasts, passages 5–10 |
This paper |
N/A |
|
| Oligonucleotides |
|
|
|
| siRNA targeting sequence CARMIL1 55604 |
Dharmacon |
On-targetplus Smartpool |
| Primers for MMP-1, MMP-3, MMP-9, MMP14 and GAPDH |
Acgt |
N/A |
| Recombinant DNA |
|
|
|
| GST-tagged deleted domain CARMIL1 constructs |
Custom DNA constructs and this paper |
LRR and CP deletions |
