FIGURE 4.

Forced swim test demonstrating immobility behavior in WMI and WLI. Early adolescent stress increases immobility behavior in adult WLIs while it reduces immobility behavior in WMI females. Immobility behavior (floating) was measured every 5s (bins) on the second day of FST. Climbing behavior was observed against the wall of the glass chamber and showed greater variations. Cohen’s d effect sizes for immobility ranged between 1.67 and 2.89. Cohen’s d effect sizes for climbing were 1.02 and 1.44. Data are presented as mean ± SEM. N = 4–10/sex/strain/stress status. *q < 0.05; ^**q < 0.01 by FDR post hoc after three-way ANOVA. ^#p < 0.05 represent independent p-values not corrected for multiple comparisons and shown for interpretation purposes.