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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: Environ Sci Technol. 2021 Jul 25;55(15):10875–10887. doi: 10.1021/acs.est.1c02656

Table 1.

ToxCast and Tox21 ER Agonism Pathway-Related Assays Used in the vAOP Modela

ID assay name assay source gene name time point
(min)		 biological process target assay design type cell line ER KE vAOP model
layer
E1 NVS_NR_hER NovaScreen ESR1 1080 receptor binding radioligand binding NA (cell-free) ER binding 2
E2 OT_ER_ERaERa_0480 Odyssey Thera ESR1 480 protein stabilization protein fragment complementation assay HEK293T receptor dimerization 3
E3 OT_ER_ERaERa_1440 Odyssey Thera ESR1 1440 protein stabilization protein fragment complementation assay HEK293T receptor dimerization 3
E4 OT_ER_ERaERb_0480 Odyssey Thera ESR1, ESR2 480 protein stabilization protein fragment complementation assay HEK293T receptor dimerization 3
E5 OT_ER_ERaERb_1440 Odyssey Thera ESR1, ESR2 1440 protein stabilization protein fragment complementation assay HEK293T receptor dimerization 3
E6 OT_ER_ERbERb_0480 Odyssey Thera ESR2 480 protein stabilization protein fragment complementation assay HEK293T receptor dimerization 3
E7 OT_ER_ERbERb_1440 Odyssey Thera ESR2 1440 protein stabilization protein fragment complementation assay HEK293T receptor dimerization 3
E8 OT_ERa_EREGFP_0120 Odyssey Thera ESR1 120 regulation of gene expression fluorescent protein induction HeLa DNA binding 4
E9 OT_ERa_EREGFP_0480 Odyssey Thera ESR1 480 regulation of gene expression fluorescent protein induction HeLa DNA binding 4
E10 ATG_ERa_TRANS_up Attagene, Inc. ESR1 1440 regulation of transcription factor activity mRNA induction HepG2 transcriptional activation 5
E11 ATG_ERE_CIS_up Attagene, Inc. ESR1 1440 regulation of transcription factor activity mRNA induction HepG2 transcriptional activation 5
E12 TOX21_ERa_BLA_Agonist_ratio Tox21 ESR1 1440 egulation of transcription factor activity β lactamase induction HEK293T transcriptional activation 5
E13 TOX21_ERa_LUC_VM7_Agonist Tox21 ESR1 1320 regulation of transcription factor activity luciferase induction VM7 transcriptional activation 5
E14 ACEA_ER_80hr ACEA Biosciences, Inc. ESR1 1920 cell proliferation real-time cell-growth kinetics T47D cell proliferation 6
a

NA: not applicable.