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. Author manuscript; available in PMC: 2021 Dec 28.
Published in final edited form as: Int J Hematol. 2020 Jun 12;112(3):395–408. doi: 10.1007/s12185-020-02904-y

Figure 5. Foreign DNA editing assay by A3Ds and A3B.

Figure 5.

(A) EGFP positive cell rates and (B) EGFP mean fluorescence intensity (MFI) compared to mock cells analyzed by flow cytometry 4 days after transfection in co-transfected HEK293T cells. Asterisks* on the error bar indicate P<0.05 (Student’s t-test). (C) Analysis of 3D-PCR products in HEK293T cells expressing A3D variants, A3B or empty as control. 3D-PCR for EGFP gene at indicated denaturation temperatures (Td) was performed. (D) Mutation frequencies in 3D-PCR products in HEK293T cells expressing A3D variants and A3B. 10 clones (4870 bps) were sequenced in each group. C/G to T/A mutation frequencies (blue bar) and mutation frequencies including all patterns (red bar) are shown. Error bars indicate S.E.M. (E) Mutation patterns of edited EGFP gene sequences derived from 3D-PCR products. (F) Dinucleotide patterns in edited EGFP gene. The rates of indicated dinucleotide sequence at C/G to T/A mutations.