Figure 2.

NEAT1 knockdown promotes cell viability, and inhibits cell apoptosis and the production of cytokines in LPS-stimulated FHCs. (A) Relative lncRNA NEAT1 expression in si-NC and si-NEAT1 transfected FHCs. (B) The MTT bioassay was performed to assess cell viability. (C) Flow cytometric analysis was performed to detect apoptosis in different groups of LPS-treated FHCs. (D) ELISA was performed to detect the levels of different inflammatory cytokines in LPS-treated FHCs. (E) Western blot analysis was performed to detect the protein expression levels of different apoptosis related proteins. All experiments were performed in triplicate. ^**P<0.01 vs. si-NC. NEAT1, nuclear enriched abundant transcript 1; LPS, lipopolysaccharide; FHCs, fetal human cells; si, small interfering; NC, negative control; TNF, tumor necrosis factor; IL, interleukin; OD, optical density.