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. 2021 Dec 10;23(2):131. doi: 10.3892/etm.2021.11054

Figure 3.

Figure 3

NEAT1 knockdown alleviates the LPS-induced cell injury by negatively regulating miR-603 expression. (A) Bioinformatics prediction of miR-603 binding sites in NEAT1. (B) Reverse transcription-quantitative PCR analysis was performed to detect miR-603 expression in the si-NC and si-NEAT1 groups. **P<0.01 vs. si-NC. (C) Relative luciferase activity of miR-NC and miR-603 mimics co-transfected with NEAT1-WT and NEAT1-MUT type. **P<0.01 vs. miR-NC. (D) Relative miR-603 expression in healthy individuals and patients with UC. (E) Pearson's correlation analysis was performed to assess the correlation between NEAT1 and miR-603 expression in patients with UC. (F) Relative miR-603 expression in LPS-treated FHCs. (G) Relative miR-603 expression in LPS-treated FHCs at different time points. All experiments were performed in triplicate; **P<0.01 vs. 0 ng/ml LPS. NEAT1, nuclear enriched abundant transcript 1; LPS, lipopolysaccharide; miR, microRNA; NC, negative control; si, small interfering; WT, wild-type; MUT, mutant; UC, ulcerative colitis; FHCs, fetal human cells.