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The IC50 values of the compounds of this work were determined with both the TNKS2 biochemical assay (quadruplicates used for each concentration tested, 95% confidence intervals are given in parentheses) and the cellular (HEK293) WNT/β-catenin signaling reporter assay (triplicates used for each concentration tested, T/F-tests were performed for the IC50 curve fitting; all p > 0.95).
