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. 2021 Nov 30;64(24):17901–17919. doi: 10.1021/acs.jmedchem.1c01212

Figure 2.

Figure 2

High-throughput screening of the novel benzodiazepinone derivative efficacy on NCX activity in the reverse mode in BHK-NCX1 and BHK-NCX3 cells loaded by Fluo-4. (A) Effect of compound 14 (10 nM and 10 μM), compound 1 (10 nM and 10 μM), and Neurounina-1 (10 nM) on NCX1 in BHK-NCX1 cells. *p < 0.05 vs control. (B) Effect of the same compounds on NCX3 in BHK-NCX3 cells. *p < 0.05 vs control, Neurounina-1, and compound 1. (C) Effect of two different concentrations of compound 4 on the NCX1 reverse mode in BHK-NCX1 cells. *p < 0.05 vs Neurounina-1 and internal control (untreated cells). The effects of compounds 1–19 on NCX1 and NCX3 reverse mode activity measured in BHK-NCX1 and BHK-NCX3 loaded by Fura-2 AM have been reported in Figure S1.